T7

Part:BBa_K921000:Design

Designed by: Eric Pederson   Group: iGEM12_Carnegie_Mellon   (2012-10-01)


T7 RNAP + IPTG->PoPs (Mutant I)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.


Source

The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.

References